Qiagen 80004 AllPrep DNA RNA Protein Mini Kit 50 Preps for Qiacube NEW (4668)

Qiagen 80004 AllPrep DNA RNA Protein Mini Kit 50 Preps for Qiacube NEW (4668)w New, old stock. Sealed in original packaging from manufacturer. First Photo is from manufacturer because box is sealed. See pictures for more detail. Manufacturer: Qiagen Model #: 80004 AllPrep DNA/RNA/Protein Mini Kit Sells for $706 New from manufacturer Description: The AllPrep DNA/RNA/Protein Mini Kit allows simultaneouspurification of DNA, RNA, and protein from the same cell or tissue sample. The kit can be used incombinationwith Allprotect Tissue Reagent, which delivers immediate stabilization of DNA, RNA, and protein in tissuesamples for long-term storage without freezing. Tissues can be efficiently disruptedusing TissueRuptor or TissueLyser system. (Taken fromManufacture website). Specifications Note* this data wastaken from an online source andmay not pertain to this particular model. Main sample type: Easy-to-lyse tissue or cells Elution volume: RNA: 30-50 l, DNA: 100- 200 L Purification of total RNA, miRNA, Poly A+ mRNA, DNA or protein: Total RNA, Genomic DNA and western-grade proteins Format: Spin column Yield: varies, depends on sample type Time per run or per prep: 60 min Processing: Manual (centrifugation) Technology: Silicatechnology, Protein precipitation Sample amount: up to 30 mg tissue or 1 x 10e7 cells Features: Maximal recovery of eachanalyze from precioussamples Time savings through short, streamlined workflow Comparable data asanalyses come fromsame sample No need for organic solvents such as phenol and acetone Applications: The Allprep DNA/RNA/Protein Mini Kit streamlines samplepreparation by enabling simultaneous purification of genomic DNA, total RNA, and total protein from the same cell or tissue sample. Total proteinpuffed using the kit is suitable for SDS-PAGE and western blotting, Genomic DNA and total RNA purified using the AllPrep DNA/RNA/Protein Mini kit deliver optimal results in all downstream applications including Real-Time PCR analysis Real-time RT-PCR analysis Performance: Genomic DNA and total RNA purified using the AllPrep DNA/RNA/Protein Mini Kit deliver optimal results in real-time PCR and real-time RT-PCR analyses. Total protein purified using the kit is suitable for SDS-PAGE and western blotting. Principle: The AllPrep DNA/RNA/Protein Mini Kit standardizes sample preparation for systemsbiology. DNA, RNA, and protein are all prepared from thesame source, eliminating the variationinherent in preparing theseanalyses fromdifferent samples. There is no need to split the sample into 3 prior to purification,allowing maximum recovery of DNA, RNA, and protein, In addition, no organic solvents such as phenol and acetone are required. Procedure: Cells or tissues are first lysed and homogenized in Buffer RLT, which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact DNA, RNA, and proteins. The lysate is then passed through an AllPrep DNA spin column. This column, in combinationwith the high-salt buffer, allows selective andefficient binding of genomic DNA. The column is washed and pure, ready-to-use DNA is then eluted. Ethanol is added to the flow-through from the AllPrep DNA spin column to provide appropriate binding conditions for RNA, and the sample is then applied to anUneasy spin column, where total RNA binds to the membrane and contaminants are efficiently washed away. High-quality RNA is then eluted in RNase-free water. Buffer APP, a novel aqueous protein precipitationsolution, is added to the flow-through of the RNeasy spin column, and the precipitated proteins are pelleted by centrifugation. Intact total proteins are redissolved in an appropriate buffer and then ready to use in downstream applications. The kit includes Buffer ALO, which is compatible with SDS-PAGE, for dissolving the protein pellet. Kit Contents: 50 AllPrep DNA Mini Spin Columns (uncolored) (each in a 2 ml Collection Tube) 50 RNeasy Mini Spin Columns (pink) (each in a 2 ml Collection Tube) 100 Collection Tubes (1.5 ml) 150 Collection Tubes (2 ml) Buffer RLT* 45 ml Buffer RW1* 45 ml Buffer RPE (concentrate) 11 ml RNase-Free Water 10 ml Buffer AW1* (concentrate) 19 ml Buffer AW2 (concentrate) 13 ml Buffer EB 22 ml Buffer APP 55 ml Buffer ALO 10 ml Handbook 1 * Contains a guanidine salt. Not compatible with disinfectants containing bleach. See page 6 for safety information. Before using for the first time, add the appropriate volume of ethanol (96100%) as indicated on the bottle to obtain a working solution. DTT must be added before use. See Things to do before starting in the protocols Manufacturer states that solutions are stable at least 6 months. We do not know date of manufacture. Intended formolecular biology applications. This product is not intended for diagnosis, prevention, ortreatment of a disease. Not forclinical use on humans. Sold as is with 30day Right of Return, only pictured items included Easy return/refund for any reason Anacapa Equipment RETURNS Anacapa Equipment Brokers, Inc. (Seller) strives to offer excellent customer service. You may return any equipment purchased from us for any reason within thirty (30) days after your receipt of the equipment for a full refund of the purchase price, provided that returned items are received in the same condition as sent. We DO NOT ship internationally. If you are using a freight forwarder then returns are VOID. 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